Toluidine blue O is used in numerous procedures, including the simple staining of Helicobacter pylori, either alone or following alcian yellow of acriflavine Schiff reagent. It is a metachromatic dye and used for staining mast cell granules, both metachromatically and orthochromatically. Please refer to the StainsFile web pages for a
full description of this dye, and for the staining method for demonstrating
Helicobacter, and one method for
mast cells.
